FastQCFastQC Report
Mon 27 Feb 2023
SRR3128128.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128128.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2143607
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279631.3044835177343608No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTGACGTGC36070.16826778416006294No Hit
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC35310.1647223581561359Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG35210.16425585473456655TruSeq Adapter, Index 22 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG31970.14914114387571978TruSeq Adapter, Index 22 (95% over 23bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA27220.12698223135117587No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23230.10836874483055896No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT102000.085.423931
CGTAGGG6600.075.487272
AGGGTAC13300.074.56635
ATAGGGC13800.073.567573
TAGGGCA11500.073.567574
ACGGGAT7200.073.113454
AGTAGGG33050.071.8177262
ACGGGTA2950.071.697214
AGGGATG28000.071.509445
GAGGGAT28350.071.123974
ATAGAGG13800.070.874261
ATAGGGA19150.069.4591453
AGTAAGG12950.068.990311
TAGGGTA7500.068.935534
TAGAGGG40300.068.461242
AAGGGTA16400.068.20954
AAGAGGG79650.067.6845252
AAGGGAC23950.067.509474
TAAGGGA21050.066.985523
AGAGGGC32450.066.048343
Produced by FastQC (version 0.11.5)