FastQCFastQC Report
Mon 27 Feb 2023
SRR3128127.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128127.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1796866
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248241.3815164848130022No Hit
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC33280.18521136244995454Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG32270.1795904647313712TruSeq Adapter, Index 22 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG26930.1498720550113364TruSeq Adapter, Index 22 (95% over 23bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTGACGTGC20280.11286317399294105No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT18300.10184398836641129No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT90200.085.617241
ACGGGTA2200.083.432624
TAGGGCA10800.076.262014
AGGGTAC9250.075.708725
TAGGGTA6900.075.030454
AGTAGGG27300.073.788242
AGGGATG22300.073.345885
GTAGGGC8300.073.148523
CGGGTAT1550.072.774775
TAGACGG2100.071.755411
GAGGGAT24600.071.170794
TAGCGGG9400.070.09812
CGAGGGA10800.069.7252663
CGTAGGG5950.069.610022
GTACGGG4350.069.246332
ATAGAGG11150.068.839341
GTAGGGT7300.068.340313
TAGAGGG31250.068.076072
AGGGTAA9750.067.969775
ATGCGGG7500.067.774842
Produced by FastQC (version 0.11.5)