FastQCFastQC Report
Mon 27 Feb 2023
SRR3128126.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128126.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241286
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143351.1548506951661421No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCACGAGTCT99920.8049716181444082No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG52430.42238452701472506No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC50200.40441928773868385No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA42970.346173242910981No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG40130.32329374535763716No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATG30010.2417653949210738No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCACGAGTCTTC21740.17514094253862528No Hit
CGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT16740.13486013698696353No Hit
ACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG14910.12011736215505532No Hit
AGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT14320.11536422709995925No Hit
AGAGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT13650.10996659915603656No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGT12440.10021864421253442No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66750.085.3158651
ACGGGTA1300.083.1476444
CCGTACA39400.080.872143
ATCCGTA40950.080.337621
TCCGTAC40800.080.1704942
CGTACAC40050.078.9728854
TACACCC40250.076.595536
GTACACC41450.076.532295
TAGGGCG1750.075.19444
TAGCGGG4650.074.790122
AGGGTAC7200.074.411125
TAGGGCA5450.073.297294
GAGGGTA8150.072.080514
TACGGGT1050.071.613713
CGAAGGG11900.071.0871352
AGGGTAT7900.070.792195
GCATAGG4200.070.597121
AAGGGTA9800.070.494744
GTACGGG2550.070.033992
AGTAAGG5200.069.692031
Produced by FastQC (version 0.11.5)