FastQCFastQC Report
Mon 27 Feb 2023
SRR3128124.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128124.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1179971
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154111.3060490469681034No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG46740.39611142985717446No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC45160.3827212702685066No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG35580.3015328342815205No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCACGAGTCT31280.2650912607174244No Hit
CGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT16660.1411899105994978No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA15450.13093542129425215No Hit
AGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT12980.11000270345627138No Hit
AGAGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT12540.10627379825436387No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT67350.086.886071
CGTAGGG3100.083.511042
ATAGCGG2150.076.638361
CGAAGGG10650.075.576962
ACGGGTA1750.075.311774
GCGAATA255.5617515E-575.1936959
TAGCGGG5050.074.566112
AGGGTAC6650.074.213745
TAGGGCA6550.073.299624
CGGGTAA1350.073.114275
GTAGCGG2200.072.7566761
GTAGGGC6050.072.3553163
GATAGGG15550.071.1345142
CGGGGTA2000.070.503055
AAGGGCA18250.070.411354
TAGGGTA5500.070.176884
AGTAAGG6650.069.3778841
AAGGGTA10400.069.247014
AGAGGGC15600.068.794413
GTAGGGT5000.068.7223
Produced by FastQC (version 0.11.5)