FastQCFastQC Report
Sat 18 Mar 2023
SRR3128123_0.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128123_0.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2071694
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293791.4181148374229013No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCATTAGACG54780.2644212900167689No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA52420.2530296462701538No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG43140.2082353861139724No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC42840.20678729580719934No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG42040.20292572165580436No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT99400.085.631311
GTACGGG4550.081.600482
CCGTACA28450.078.467233
TCCGTAC29650.077.3521042
ATCCGTA29900.076.9516751
AGGGTAC13600.076.0257645
CGTACAC29400.075.6120154
TAGGGTA7150.075.5907754
TAGGGCA11950.074.331164
GTAGGGA15350.072.563293
GAATAGG12600.072.445531
AGTACGG3350.071.631781
AGTAGGG30350.071.3870242
GGTAAGG11100.070.790331
ATAGGGA17000.070.496613
TAAGGGA13950.070.0754853
AGGGATG21500.069.512945
AAGGGTA16050.069.398544
ATAGAGG15200.069.030611
TAAGCGG5350.068.599551
Produced by FastQC (version 0.11.5)