FastQCFastQC Report
Mon 27 Feb 2023
SRR3128122.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128122.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2301386
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350191.5216482589187559No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC49570.21539194207316806No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG49520.21517468169181528No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG43440.18875581931931454No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA35470.15412451453167786No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCATTAGACG30890.1342234635997612No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT118500.087.0248641
ATAGGGA20000.077.083123
ATAGCGG4100.074.550841
GTACGGG6600.074.06362
TAGGGTA9100.072.827124
CGTAGGG6400.071.9716642
AGTAGGG38000.071.863442
TAGGGCA12200.071.658644
AGGGTAC14150.070.087655
GTAGGGA17800.069.974743
TAAGGGA18150.068.884333
GGTAAGG12900.068.5316541
AGGGCAT21200.068.285785
TAGAGGG43950.068.2302862
TAGCGGG11650.068.1830142
AGGGATG30500.067.8060155
AAGGGTA17200.066.9503864
TACGGGG9200.066.926623
ATAGGGC12650.066.880173
AAGAGGG81700.066.159342
Produced by FastQC (version 0.11.5)