FastQCFastQC Report
Mon 27 Feb 2023
SRR3128121.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128121.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2012523
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307741.5291253814242123No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG40110.1993020700881431No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC39560.19656918206649066No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG37640.18702891842726765No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT102100.086.634661
ACGGGTA2800.075.633724
GTACGGG4750.075.297562
AGTAGGG35000.074.3563462
ATAGGGA17050.073.144633
TAGGGCA10650.072.469484
AGTAAGG11400.071.438421
GTAGGGC8650.071.271543
GTAGGGA18400.070.847243
AGACGGG13050.070.321012
TAGGGTA7900.070.293614
ATAGAGG14400.070.285641
AAGGGTA16250.070.0846564
AAGAGGG70900.069.7617652
GAGGGAT24850.068.934394
AGGGATG24850.068.847045
TAGAGGG37550.068.554872
AGGGTAC13850.067.871975
TATAGGG15050.067.542672
AGTACGG3700.067.43181
Produced by FastQC (version 0.11.5)