FastQCFastQC Report
Mon 27 Feb 2023
SRR3128120.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128120.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7101305
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3235564.5562893017551005No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT266180.3748325131789157No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCT159340.22438129329749953No Hit
CGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCT158040.22255064386052986No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTT146200.20587765206535985No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT106100.14940915789421805No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT105840.1490430280068241No Hit
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA86370.12162553220851662No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG85910.12097776394620426No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82280.11586602744143505No Hit
ATGATGGTTACAATGAAGGAGGAAATTTTGGCGGTGGTAACTATGGTGGT74920.10550173524443746No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTC72730.10241779503908084No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT903050.090.583091
ATAGGGC33750.072.2980653
ATAGGGA46950.070.633023
AGGGACT147750.069.6575
GGTAAGG34000.069.591971
AGATAGG36450.069.052291
GACTTAA109450.068.141138
TAGTAGG22950.067.979761
GTTTTTT1211400.067.80832
TAGGGAT30200.067.382554
GATAGGG97600.066.266562
ACTTAAT112300.066.2444159
AGTAGGG103700.066.002532
AATAGGG86800.065.720092
AAGGGGC235450.065.4013754
ATAGAGG42350.065.219661
GGACTTA116300.065.1378567
GGATAGG38200.065.0251851
TATAGGG45950.064.174512
AGTAAGG41950.063.706751