FastQCFastQC Report
Mon 27 Feb 2023
SRR3128119.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128119.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4867129
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1966554.040472319513208No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT168230.34564524589342094No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCT167750.3446590382132875No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTT160840.3304617568180338No Hit
CGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCT157240.3230651992170333No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC104350.21439744046233417No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG87770.1803321835110596No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTC79160.16264208324866672No Hit
CGTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTC73660.15134178691380484No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT64250.13200800718452296No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT62600.12861791828406438No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC54160.11127709990838541No Hit
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA53900.11074290408164648No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGC49220.10112737920034583No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT569900.090.745241
AGGGACT96500.070.959135
GACTTAA70350.070.546578
ATAGGGA30400.069.7256243
TAGTAGG15600.069.63631
AGATAGG24400.069.5771
ACTTAAT71550.069.2977149
GTTTTTT755000.068.576512
GGTAAGG23350.068.274911
AGTAGGG67400.067.641032
ATAGGGC24250.067.639933
GGACTTA74850.067.498287
AGTAAGG28600.067.087631
TAGGGAC20250.067.073874
TAGGGAT20200.067.007234
ATAGAGG29250.065.275241
AAGGGGC161350.065.217734
AATAGGG60050.064.8843462
GGATAGG25350.064.557931
GAGGGAT39550.064.28824
Produced by FastQC (version 0.11.5)