FastQCFastQC Report
Mon 27 Feb 2023
SRR3128118.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128118.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7093308
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3021634.259831943008819No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT268180.3780746585373143No Hit
CGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCT164040.23126022442561356No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCT159870.22538144403147303No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTT150220.21177707213615987No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT106080.14954940628547359No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT105340.14850616947692108No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG89050.12554086189405564No Hit
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA85490.12052204697723544No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75110.10588853606807994No Hit
ATGATGGTTACAATGAAGGAGGAAATTTTGGCGGTGGTAACTATGGTGGT74930.1056347757632969No Hit
CGTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTC73710.10391484480865629No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTC72810.10264604328474106No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT852500.090.762541
ATAGGGC32900.072.2805253
AGGGACT143850.071.679255
TAGTAGG23300.068.863971
GACTTAA108600.068.807218
AGATAGG37800.068.464351
ACTTAAT108950.068.370499
GGTAAGG34900.067.816611
GTTTTTT1151550.067.611432
AGTAGGG101900.067.459322
ATAGGGA47100.067.451643
ATAGAGG44300.066.703631
GTAGGGA48300.065.970413
GGACTTA115000.065.713557
TAGGGAT30850.065.201164
TAGGGAC31200.064.620364
AAGGGGC231900.064.405044
AATAGGG88150.063.7403372
ATAAGGG81450.063.557242
GATAGGG97400.063.5280762
Produced by FastQC (version 0.11.5)