FastQCFastQC Report
Mon 27 Feb 2023
SRR3128116.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128116.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10474853
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4075143.8904030443195716No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT428910.40946636673564774No Hit
CGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT213580.20389784944953404No Hit
CGTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTT197450.18849906533294547No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCT197340.18839405192607475No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT176900.1688806515948243No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG172340.1645273685463653No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT143960.13743390957371907No Hit
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA116390.11111373114257546No Hit
ATGATGGTTACAATGAAGGAGGAAATTTTGGCGGTGGTAACTATGGTGGT110380.1053761804580933No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1168250.090.80141
AGGGACT237950.072.344185
ATAGGGC47900.071.877513
ATAGGGA70600.070.715133
ACTTAAT177500.070.2940449
GACTTAA178700.070.11138
AGTAGGG154550.069.42282
TAGTAGG34800.068.788851
GGACTTA187050.067.860847
GGTAAGG55400.067.537391
GTTTTTT1582750.067.339592
ATAGAGG68000.066.249491
GTAGGGA73650.065.804233
AGATAGG51100.065.750971
TAGGGAT48300.065.723994
AAGGGGC343500.065.705434
AGTAAGG61950.065.340941
TATAGGG72050.065.3053742
GGATAGG56650.065.2985151
TAGGGAC48400.065.296264