FastQCFastQC Report
Mon 27 Feb 2023
SRR3128115.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128115.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7182339
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2477623.4496004713784743No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT256650.357334845932502No Hit
CGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT211180.29402677874157707No Hit
CGTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTT210390.29292685850667866No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCT202090.28137073451977135No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG167420.2330995515527741No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC139410.1941011138571989No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT105190.1464564677328653No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTC102050.14208463287516784No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC101910.14188971030189468No Hit
CGTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTC88060.12260629858880233No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT84390.11749654256085655No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGC74090.10315581038433301No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT743050.090.940421
ATAGGGC31850.073.930313
AGGGACT146050.071.532765
GACTTAA105950.070.215818
ACTTAAT105750.069.859779
TAGTAGG22550.068.2087861
ATAGAGG44500.067.9657441
GTTTTTT992850.067.963752
AGTAGGG104000.067.923952
GGTAAGG37800.067.568951
AGATAGG36500.067.5270161
GGACTTA111700.067.4025657
ATAGGGA44100.067.355453
GTAGGGA50450.066.423643
AATAGGG86750.065.881192
GGATAGG37150.065.712451
TATAGGG45100.065.3413852
AAGGGGC231250.064.993944
AGTAAGG38050.064.776241
GATAGGG92450.064.767912