FastQCFastQC Report
Mon 27 Feb 2023
SRR3128114.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128114.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10524806
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3851813.6597444171417504No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT431030.4095372399263226No Hit
CGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT225170.21394218572769894No Hit
CGTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTT204030.19385630480979887No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCT200160.19017927741375945No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT178280.16939029565010508No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG173140.16450659518094682No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT141760.13469131877585203No Hit
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA116290.11049134777401123No Hit
ATGATGGTTACAATGAAGGAGGAAATTTTGGCGGTGGTAACTATGGTGGT112090.10650077540621651No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC108480.10307078344246916No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1140100.090.794731
ATAGGGC48100.074.159343
AGGGACT228600.071.338245
TAGTAGG36150.070.2919851
AGTAGGG149750.069.7981642
GGTAAGG58950.069.447371
GACTTAA177350.069.1637348
ATAGGGA70700.069.132633
GTAGGGA71800.068.269863
TAGGGAT47250.067.834674
AGATAGG55000.067.7614751
ACTTAAT181150.067.505339
GTTTTTT1551250.067.034162
TAGGGAC50300.066.617874
GGACTTA188300.066.314797
ATAGAGG68050.065.6925051
AAGGGGC343300.065.450674
AATAGGG132000.065.3276142
TATAGGG69650.065.009452
AGTAAGG58400.064.380491