FastQCFastQC Report
Mon 27 Feb 2023
SRR3128113.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128113.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12481524
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4743513.8004253326757214No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT518440.41536594409464744No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCT221340.1773341140072318No Hit
CGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT219170.17559554426206286No Hit
CGTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTT218290.17489050215342292No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT218280.1748824903112793No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG210930.16899378633570708No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT177500.14221019804953305No Hit
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA138590.11103612026864668No Hit
ATGATGGTTACAATGAAGGAGGAAATTTTGGCGGTGGTAACTATGGTGGT134580.10782337156904878No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC127790.10238333075352016No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124910.1000759202161531No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1369150.090.283731
ATAGGGC57500.074.81613
AGGGACT281050.071.398015
GACTTAA211500.070.5922858
ACTTAAT214000.069.3503659
AGTAGGG184250.069.259162
TAGTAGG43850.069.202931
ATAGGGA88100.068.148583
GGACTTA223300.068.1036157
GGTAAGG68750.067.7480551
GTAGGGA90850.067.379523
GTTTTTT1847950.067.169512
AGATAGG64900.067.055031
TAGGGAT57400.066.756384
AAGGGGC410050.066.514014
TAGGGCA42150.065.8114554
TATAGGG89700.065.680062
AATAGGG154750.065.386262
GGATAGG67200.065.250071
AGTAAGG71650.065.005981