FastQCFastQC Report
Mon 27 Feb 2023
SRR3128112.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128112.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1676039
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT668693.989704296857054No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCT49740.2967711371871418No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTT49480.2952198606357012No Hit
CGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT43210.257810229952883No Hit
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG26730.1594831623846462No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTC23170.1382426065264591No Hit
CGTCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTC20430.1218945382535848No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT18560.11073727997976181No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC17500.10441284480850385Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT181500.090.71921
ATAGGGC9500.078.161873
AGGGATG21300.071.70765
ATAGCGG3450.070.927741
TAGGGAT8350.070.3533864
AGTAGGG24050.070.181442
AGATAGG7450.069.481361
GAATAGG8300.069.169341
ATAGGGA12650.068.729423
TAGGGCA6100.067.0271764
AGAGGGC23300.066.762643
AATAGGG21500.066.6966862
TAATAGG5100.066.434591
GTTTTTT252000.066.064522
AGGGAAT16250.065.360685
GATAGGG22400.065.276262
ACGGGAT4050.064.982214
GTAGGGA10150.064.822153
TATAGGG13900.064.60432
AGGGCAT12400.064.051085