FastQCFastQC Report
Mon 27 Feb 2023
SRR3128111.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128111.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1579419
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT617863.9119448354109956No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTT54750.3466464567033827No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCT53540.3389854117241846No Hit
CGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT45560.288460503514267No Hit
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG29180.18475148139917272No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTC24460.15486707453816878No Hit
CGTCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTC22690.1436604219652923No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC19950.12631227052479424Illumina Single End Adapter 1 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT16330.10339245000851578No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT167000.090.313971
ATAGGGC10350.072.656563
TAGGGCA6350.071.792574
ATAGGGA11400.071.324133
AGTAGGG24800.070.689092
AGGGCAT9850.070.14175
AGGGATG19300.069.403725
GCATAGG3850.068.4282151
AGATAGG8400.068.3263851
GAATAGG7900.067.291351
AGAGGGC24450.067.087533
TAAGGGA11300.066.9641953
ATAGAGG9850.066.387521
GTTTTTT229950.066.01832
GTACGGG3000.065.79962
AAGGGTA9000.065.2753144
CGTTTCT9700.064.504171
GGGCGAT7800.064.469896
TAGAGGG27200.064.451812
GAGGGAT16000.064.328824