FastQCFastQC Report
Mon 27 Feb 2023
SRR3128109.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128109.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2292921
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174860.7626080444986985No Hit
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG48000.20933996417669865TruSeq Adapter, Index 23 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG40890.17833148198302515TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC39320.17148432065474561Illumina Single End Adapter 1 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38450.16769003380404296No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT55550.082.597041
CGTAGGG6400.074.9415742
GTAGGGT9800.073.373733
ATAGGGC13250.072.71323
TAGGGAC8500.070.77284
TAGGGCA11350.070.392824
ATAGAGG17650.069.855481
AGTAGGG34900.069.253282
AATAGGG29450.067.858812
TAGGGTA8000.067.558994
ATAAGGG27300.066.485552
AGGGCAT19300.066.478415
TAGGGTC6300.066.393374
AGGGAAT26150.066.1380545
ATAGGGA19550.065.86873
AGTAAGG14050.065.648321
TAGAGGG40500.065.134412
AGGGATC18600.064.684815
AGGGTAC9500.064.312455
TAGGGCG3950.064.2498254
Produced by FastQC (version 0.11.5)