FastQCFastQC Report
Mon 27 Feb 2023
SRR3128108.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128108.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118288
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158970.7504645260701094No Hit
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG53610.253081733928531TruSeq Adapter, Index 23 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG42930.20266366046543244TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC41720.1969515004569728Illumina Single End Adapter 1 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33490.15809937081265626No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT49200.081.731431
CGTAGGG5400.073.1032
ATAGGGC13250.073.063593
AGTAGGG30200.071.7370762
ATAGCGG4450.070.811351
ATAGAGG14950.069.5247041
GTAGGGT7450.069.388273
GGTAAGG11550.069.223731
TAGCGGG10950.067.3806462
TAGGGTA6900.066.7462164
AGGGAAT23200.066.444045
ATAGGGT8300.066.245653
AGGGACT27850.066.150185
TAGGGAC8750.066.061244
TAGGGAT11900.065.950674
AAGAGGG67200.065.806692
ATAGGGA17450.065.71193
AATAGGG27750.065.3693162
ACGGGAT4750.065.298014
GAATAGG12550.065.206911
Produced by FastQC (version 0.11.5)