FastQCFastQC Report
Mon 27 Feb 2023
SRR3128107.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128107.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2608391
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230320.8829964526023897No Hit
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG51460.19728637309360444TruSeq Adapter, Index 23 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT44400.1702198788448511No Hit
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG43700.16753623210630614TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC42830.1642008425884003Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74450.082.2674561
ATAGGGC13600.075.336933
CGTAGGG7400.072.405542
TAGGGAC12100.071.081414
TAGGGCA12750.070.775244
AATAGGG35550.069.541622
TAGGGTC7850.069.451034
AGTAGGG41500.069.1977162
AAGGGTA15450.069.053824
GGTAAGG14450.069.016011
ATAGGGT9800.068.100863
TAAGGGT9750.067.968053
ATAGGGA22900.067.933323
AGGGAAT29750.066.825735
GTAGGGT11400.066.788323
ATAGCGG5800.066.507041
TATAGGG21500.066.455942
ATAAGGG36850.066.45062
GTAGGGA20550.065.638773
TAGAGGG42900.065.18662
Produced by FastQC (version 0.11.5)