FastQCFastQC Report
Mon 27 Feb 2023
SRR3128106.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128106.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2158491
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187240.8674578675565475No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG44360.205513944695623No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG33860.15686884958056346No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC32290.1495952496443117No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24500.11350522193513894No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22030.10206204241759638No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT1500.081.463413
CGTTTTT54000.079.48141
ACGGGTA2300.077.649084
AGGGAAT23450.071.348965
TAGGGCA9450.071.118854
AGTAGGG31100.070.610372
GGTAAGG11500.070.387421
ACGGGAT6100.070.111954
GAATAGG10600.069.704081
TAGGGTA8700.069.6868744
ATAGCGG3700.068.684141
GGATAGG12000.067.846791
AGGGTAC10700.067.642165
AGAGGGC29900.067.589273
CGTAGGG4950.067.447392
GTAGGGT10050.067.34063
ATAAGGG25250.067.229182
AATAGGG28200.066.699542
AAGGGCA28400.066.6909944
TAAGGGA15900.066.211193
Produced by FastQC (version 0.11.5)