FastQCFastQC Report
Mon 27 Feb 2023
SRR3128105.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128105.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2024081
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170610.8429010499085757No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG49780.24593877418937288No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC36400.17983470029114448No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG36210.17889600267973466No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT21160.10454127082858838No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT47800.080.093971
AGTAGGG28000.074.18272
CGTAGGG4200.072.7281342
ACGGGAT4350.072.380894
AGGGAAT21300.071.262545
AGAGGGC28250.070.864633
GTACGGG3450.070.830882
ATAGCGG3000.070.549611
GTAGGGT8350.070.349653
ACGGGTA2500.069.5504764
TAGGGTA7800.068.68294
TAGGGCA9100.068.68294
AGGGTAC10200.066.804585
AGGGTAT9900.066.455555
ATAGAGG10900.066.450391
GAATAGG10550.065.980041
AGGGATG20200.065.604875
AAGAGGG64500.065.353842
AAGGGTA15350.065.2093054
TAAGCGG4400.065.204931
Produced by FastQC (version 0.11.5)