FastQCFastQC Report
Mon 27 Feb 2023
SRR3128104.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128104.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2492315
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249341.0004353382297182No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG50390.20218150594928813No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG39180.15720324276826966No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC39050.15668163935939075No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30000.12037001743359085No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27320.10961696254285673No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70350.080.642291
CGTAGGG6150.073.3655552
AGGGAAT26300.072.912255
AGTAGGG36150.071.3773042
AAGGGTA18100.070.889224
AGGGCAT18250.070.8216255
ACGGGAT6150.069.544434
TAGGGCA10450.069.262884
GTACGGG4950.068.363362
TAGACGG3800.068.086141
AGGGTAC14500.067.744555
ATAGAGG15450.067.5933761
GAATAGG12000.067.03391
GTAGGGT11150.067.022153
ATAAGGG31600.066.781372
ATAGGGC14100.066.66643
AAGGGCA32650.066.3611454
AGGGTAT13450.066.044345
TAGAGGG40300.065.426542
GGGCGAT9800.065.224226
Produced by FastQC (version 0.11.5)