FastQCFastQC Report
Mon 27 Feb 2023
SRR3128103.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128103.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925163
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104511.1296387771668344No Hit
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG45720.4941831871789079TruSeq Adapter, Index 27 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC41830.4521365424254969TruSeq Adapter, Index 27 (100% over 24bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC41430.4478129799829868No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG40280.43538273796077015TruSeq Adapter, Index 27 (95% over 24bp)
AAAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT20590.22255537672820896No Hit
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT17060.18439993817305708TruSeq Adapter, Index 27 (100% over 22bp)
AGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT14370.15532398074717646TruSeq Adapter, Index 27 (100% over 22bp)
TAGACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT13670.1477577464727837No Hit
GGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT12670.1369488403665084TruSeq Adapter, Index 27 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG10920.11803325468052657TruSeq Adapter, Index 27 (100% over 23bp)
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCCAC10250.1107912875893221No Hit
GGAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT9450.10214416270430184TruSeq Adapter, Index 27 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT9280.10030664866623504TruSeq Adapter, Index 27 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT1100.089.718035
AGGGTAC6000.085.374555
CGCTACG8050.085.233473
CGTTTTT32300.084.5200651
GGGTACG1450.084.267196
AGGGAAT21800.082.349325
CGTAGGG2000.082.277112
AGGGATG21700.081.862545
GCTACGA8400.081.682074
ACGGGTC1500.081.458284
ACGGGTG1850.081.288934
GTCGAGG1450.081.153331
TCCGCTA8500.080.847821
TACGCGG353.824789E-880.6896061
GGATGGC17750.080.222737
CTACGAC8650.079.321315
GGGAATG28150.078.9652256
CGAAGGG7000.078.583042
GGGCGAT2900.077.7850956
AAGGGTA9550.077.751164
Produced by FastQC (version 0.11.5)