FastQCFastQC Report
Mon 27 Feb 2023
SRR3128102.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128102.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences869282
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92881.068468000027609No Hit
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG50860.5850805607386326TruSeq Adapter, Index 27 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC44840.5158280051812876TruSeq Adapter, Index 27 (100% over 24bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG44740.5146776305042553TruSeq Adapter, Index 27 (95% over 24bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37860.43553185272443234No Hit
AAAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT22570.25963956460619225No Hit
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT18070.20787270413973832TruSeq Adapter, Index 27 (100% over 22bp)
TAGACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT15700.1806088242940726No Hit
AGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT15100.1737065762318787TruSeq Adapter, Index 27 (100% over 22bp)
GGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT13740.1580614806242393TruSeq Adapter, Index 27 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG11680.13436376227737376TruSeq Adapter, Index 27 (100% over 23bp)
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCCAC10600.12193971576542481No Hit
GAATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT10130.11653295478337294TruSeq Adapter, Index 27 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT10010.11515250517093417TruSeq Adapter, Index 27 (100% over 22bp)
GGAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT9860.1134269431553857TruSeq Adapter, Index 27 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG9590.11032093152739848TruSeq Adapter, Index 27 (95% over 24bp)
AGAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT9480.10905551938266295TruSeq Adapter, Index 27 (95% over 21bp)
GGAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT9100.10468409560994016No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA900.088.776374
ACGGGTC750.087.731944
CGTAGGG2150.087.440472
CACGGGT600.086.16533
CGCTACG7100.085.3930053
CGTTTTT28950.085.290681
TCCGCTA7550.082.850581
AGGGAAT18100.082.832935
CTACGAC7350.082.488485
GCTACGA7350.082.488484
AGGGCGA3150.082.062195
CGAAGGG6150.081.771052
AACGGGT1900.081.630283
AAGGGTA7400.081.2960054
AGTAAGG4300.080.938311
CAAGGGT3900.080.74233
AGGGTAC5300.080.696845
AAGGGAT19100.079.726474
TAAGGGA8100.079.492563
AGGGATG18150.078.979465
Produced by FastQC (version 0.11.5)