FastQCFastQC Report
Mon 27 Feb 2023
SRR3128101.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128101.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1043589
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136741.310285945903991No Hit
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG48260.4624425899468086TruSeq Adapter, Index 27 (95% over 24bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC47600.45611826111620574No Hit
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC47270.45295609670090425TruSeq Adapter, Index 27 (100% over 24bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG45190.43302487856809524TruSeq Adapter, Index 27 (95% over 24bp)
AAAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT23340.22365126500950086No Hit
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT17550.16816965299557585TruSeq Adapter, Index 27 (100% over 22bp)
TAGACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT16290.1560959343189704No Hit
AGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT15620.1496757823242675TruSeq Adapter, Index 27 (100% over 22bp)
GGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT12350.11834160766355337TruSeq Adapter, Index 27 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG11630.11144233984835025TruSeq Adapter, Index 27 (100% over 23bp)
ACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT10940.10483054152544728TruSeq Adapter, Index 27 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA3600.087.4742365
CGCTACG7500.084.597883
CGTTTTT44400.084.531621
TAGGGTC2300.083.780524
AACGGGT3100.083.385013
AGGGTAC6600.083.320095
TAGGGCG1650.082.6040044
GCTACGA7750.081.868924
TAGGGTA5050.080.968274
CGAGGGA5450.080.199833
CGTAGGG2700.080.0720752
AGGGTAA6250.079.713845
GAGGGTA8500.079.6215444
GGGTATC5700.079.1597066
AGGGATG26550.079.131765
CTACGAC8150.078.431255
AAGGGTA9950.078.4100954
GGATGGC22900.078.19837
AGGGAAT21900.078.1205145
CTAGGGT1700.077.409833
Produced by FastQC (version 0.11.5)