FastQCFastQC Report
Mon 27 Feb 2023
SRR3128100.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128100.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2484242
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219900.8851794631923944No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35620.1433837766207962No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG30450.12257259960986087TruSeq Adapter, Index 23 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26910.10832278014782779No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71500.082.550411
ATAGCGG5550.075.478751
ACGGGAT6000.075.193944
TAGGGTA8800.074.766714
GAATAGG15500.073.790821
ATAAGGG38100.070.581572
GAGGGAT31100.070.418774
TACGGGT1750.069.8229453
ATAGGGC13800.069.4726643
TAGAGGG45850.069.4175952
AGGGATG38500.069.334695
AGGGAAT24350.068.902055
ATAGGGA24700.068.686783
AGTAGGG43350.068.106852
TAAGGGA20950.067.9706653
AAGGGTA18100.067.508384
TAGGGCA9900.067.4087144
AGGGTAC13750.066.9909745
TAGGGAT19300.066.720024
AGGGATA24550.066.617865
Produced by FastQC (version 0.11.5)