FastQCFastQC Report
Mon 27 Feb 2023
SRR3128099.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128099.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2310959
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196010.8481760169695785No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33020.14288440426680005No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG32220.1394226379611235TruSeq Adapter, Index 23 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG26760.11579608292488097TruSeq Adapter, Index 23 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC24070.10415589372204354TruSeq Adapter, Index 20 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24060.10411262164322257No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT59400.081.468891
TAGGGTA7700.076.901094
ATAGGGC14000.075.863533
TAGGGCA10000.075.6621254
GAATAGG13850.074.363061
TAGACGG2950.073.332871
ATAGCGG4650.071.807241
ATAGGGA21250.071.653723
ACGGGAT5750.070.288344
ACGGGTA3350.070.141954
TAGAGGG39900.069.60932
ATAAGGG33700.069.028432
AGTAGGG41100.068.720342
AGGGATG34050.068.1838155
TAAGGGA18750.068.174243
AATAGGG39800.067.776862
ATAGAGG17050.067.577881
TAGGGAT15550.067.09274
AGTAAGG15300.067.0081941
AAGGGTA16350.066.971634
Produced by FastQC (version 0.11.5)