FastQCFastQC Report
Mon 27 Feb 2023
SRR3128098.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128098.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2892594
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284630.9839956800021019No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC44760.15474000153495443No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG31620.10931364719694503TruSeq Adapter, Index 23 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31120.10758509490097815No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81000.082.288751
ATAGCGG6800.075.400331
GAATAGG18000.074.739331
TAAGGGA23600.070.698713
ATAGGGA30700.070.57613
AGGGATG44850.070.316175
AAGGGTA22650.070.136434
ACGGGAT8250.069.5025944
TAGGGCA12450.069.461414
GTACGGG5650.069.0438162
AGTAGGG50100.068.7640462
GAGGGAT33350.068.4913254
TAGGGTA9900.068.3632054
AGGGAAT32750.068.167515
AGTAAGG20800.067.844331
TAGAGGG49150.067.798162
AGGGTAA16450.067.428155
ATAGGGC16100.067.142443
TAGGGAT21600.067.0181054
AATAGGG48400.066.7123952
Produced by FastQC (version 0.11.5)