FastQCFastQC Report
Mon 27 Feb 2023
SRR3128097.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128097.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840737
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95891.1405469249004148No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG46310.5508262393590386No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG42260.5026542188579782No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC39290.46732807049053393No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC20290.2413358755472877No Hit
AGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT16010.19042816005480906No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT14700.1748465929297747No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT13770.16378486970360528No Hit
GGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT12790.15212843017495364No Hit
ACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG12210.1452297210661598No Hit
AGAGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT11120.13226490567204727No Hit
ACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT10830.12881555111765036No Hit
TCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG9520.11323398399261601No Hit
TAGACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCT9260.11014145921970842No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT9230.1097846294382191No Hit
GCCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT9070.10788153727027597No Hit
GAAGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT8680.10324275011091459No Hit
GGAACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT8660.1030048635899217No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAT353.583409E-1093.9963158
ACGGGTA1000.089.296494
ATTGCGG1200.086.2710341
CGTAGGG1600.085.2196352
CGTTTTT28450.084.8513261
TAAGGGA8600.084.705983
ATCGCGG500.084.702471
AGGGTAT6850.083.704745
CGAAGGG5000.082.7512052
CGCTACG4150.082.6714553
AGGGAAT19900.082.4239045
ATAGCGG1500.081.5653461
GTAGCGG1050.080.669021
AGGGATG20550.080.502935
GAGGGTA4850.080.429834
TCCGCTA4350.080.050871
GGATGGC14500.078.762437
AGTACGG900.078.428211
GATCGAT301.4561392E-678.330269
CGGGAAT2100.078.330265
Produced by FastQC (version 0.11.5)