FastQCFastQC Report
Mon 27 Feb 2023
SRR3128096.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128096.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences796386
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85801.0773670054471072No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG50770.6375049285145645No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG47020.5904172097450231No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC43830.5503612569783999No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC18790.23594086284791543No Hit
AGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT16590.20831606783645118No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT16180.20316781058431466No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT15840.1988985240825429No Hit
GGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT14350.18018900382477843No Hit
ACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG12640.15871700406586756No Hit
ACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT11870.14904832581185506No Hit
AGAGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT11680.14666254806086496No Hit
TAGACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCT11030.1385006768074778No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT10540.13234788155492436No Hit
TCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG10320.12958540205377794No Hit
GCCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT9860.12380930855138086No Hit
GAAGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT9720.12205136705065132No Hit
GGAACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT9420.11828434954908801No Hit
GGAGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT9110.1143917647974726No Hit
AGGCCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT8490.10660659529424174No Hit
AATGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT8390.10535092279372064No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCG1150.085.81484
CGTTTTT25300.084.403021
CGGGTAC900.083.5498055
TAGACGG1150.081.8002851
AGGGATG17250.081.461065
AGGGAAT17750.081.2845465
ATTGCGG1400.080.631711
TAAGGGA7600.080.3841553
CGAGGGA3950.079.711023
CGGGATG3650.078.542545
AGGGTAC3850.078.124495
AGGGCGA4100.077.945865
GCGGGAT3750.077.696444
AGGGCAT5150.077.567485
AAGGGTA6750.077.2787254
GGATGGC12900.077.230157
GATAAGG4450.077.1588061
GAGGGAT11650.077.045664
GATGACC8850.076.9955148
TCCGCTA3300.076.966631
Produced by FastQC (version 0.11.5)