FastQCFastQC Report
Mon 27 Feb 2023
SRR3128095.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128095.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences928917
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125871.3550188014645013No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG46730.5030589385273389No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG46440.4999370234369701No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC42110.453323601570431No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24390.26256382432445524No Hit
AGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT17400.1873149054221206No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT15730.16933698059137683No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT14920.16061714878724365No Hit
GGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT12430.1338117399078712No Hit
AGAGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT12040.1296133023725478No Hit
ACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG11910.12821382319410668No Hit
TAGACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCT11180.12035520934593726No Hit
ACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT10910.11744859874455951No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT10640.11454198814318178No Hit
TCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG10350.11142007305281311No Hit
GCCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT9370.1008701530922569No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATT156.9032225E-493.995118
CGTTTTT36800.085.425731
AGGGATG25000.083.467645
CGTAGGG1800.080.944592
AGGGCAT7550.080.9229355
TAAGGGA9550.080.220033
AAGGGTA7750.080.051754
GGATGGC17500.080.030117
AGGGAAT23600.079.4577265
AGGGTAC4150.079.272985
AGGGTAT6250.078.955885
TAACGGG3100.078.8388442
CGGGTAC900.078.3292545
GAAGGGT7250.077.144963
AGGGCTA3550.076.784735
AAGGGCA11700.076.726624
GAGGGAT16800.076.654894
AGTAAGG4550.076.5385741
GATGGCA16100.076.4804848
CGAGGGA4700.076.000133
Produced by FastQC (version 0.11.5)