FastQCFastQC Report
Mon 27 Feb 2023
SRR3128093.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128093.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2152380
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT737863.4281121363328038No Hit
CGTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTT89650.4165156710246332No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCT82820.38478335609883013No Hit
CGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT74430.3458032503554205No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG45780.21269478437822317TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTC38370.17826777799459204No Hit
CGTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC35440.16465494011280535No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC29050.13496687387914771TruSeq Adapter, Index 21 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25680.11930978730521562No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT214050.090.0968251
ATAGGGC12550.074.137573
TAGGGTA8150.073.225624
AGGGATG31200.070.337895
ACGGGAT6450.068.483364
AGGGTAC9350.068.3524865
ATAGCGG4350.068.122751
AAGGGTA14950.067.89374
AGAGGGC31500.067.727063
AGGGAAT22250.067.162085
TAGGGCA9000.066.831994
GTAGGGT9200.066.4006653
AGGGTAA11850.066.225485
TAGGGTC5900.066.106324
AGTAAGG10250.066.0814361
ATAGAGG13100.065.708361
AGTAGGG33150.065.490052
ATAGGGA18950.065.465373
AAGGGAT30400.065.385844
AGGGACT23500.065.189365