FastQCFastQC Report
Mon 27 Feb 2023
SRR3128092.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128092.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2668918
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1034883.8775263983382033No Hit
CGTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTT87210.32676163149261234No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCT84380.3161580835379731No Hit
CGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT73210.27430591722937914No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG48080.1801479101268754TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTC40390.15133473564942798No Hit
CGTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC36320.13608511014575944No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32610.12218434586600262No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC30700.11502788770580438TruSeq Adapter, Index 21 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT293550.090.1381451
ATAGGGC16750.074.6401753
AGGGATG41600.073.097165
GTAGGGT9500.070.7486653
AGAGGGC36350.070.727573
TAGAGGG44900.068.9834442
AGTAGGG40300.068.926822
ATAGAGG16900.068.763141
GAGGGAT31350.067.1655044
CGTTTCT13500.066.564861
AGGGTAC11200.066.302315
TAGGGTA9350.065.851484
GTTTTTT403450.065.739462
GGGCGAT12950.065.6898656
ATAGGGA23500.065.6012043
AGGGTAT14800.065.417665
AGGGAAT30600.065.276535
AAGGGTA19750.065.206264
GTAGGGA21450.065.078123
TAGGGCA13150.065.050624