FastQCFastQC Report
Mon 27 Feb 2023
SRR3128091.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128091.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2902559
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT379351.3069501774124144No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT48500.16709393331884037No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT115750.084.6326751
ATAGGGC15700.074.834363
TAGGGCA13200.070.137934
ATAGAGG19000.069.373151
AGTAGGG49400.069.30142
AGGGATG39850.069.108275
TATAGGG23800.068.167872
AGGGTAC16650.068.024215
ATAGGGA24450.067.851043
GAATAGG17150.067.5239641
TAGGGTC8850.067.440554
ACGGGTA3700.067.3185654
AGATAGG18700.067.213521
ATAGGGT12900.066.668723
AGAGGGC41800.066.446473
GTAGGGT12950.066.41133
GAGGGAT26000.066.336634
AAGAGGG99550.065.85052
ACGGGAT7650.065.732934
TAGAGGG53200.065.2352452
Produced by FastQC (version 0.11.5)