FastQCFastQC Report
Mon 27 Feb 2023
SRR3128090.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128090.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2780733
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT349771.2578338157600892No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT45780.16463285040311312No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC32590.11719931399382824No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG31290.1125242876608434TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG31040.11162524413526936TruSeq Adapter, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT104750.084.6914751
AGGGTAC15200.072.350495
ATAGGGA22450.071.803723
ATAGGGC14900.071.283883
AGTAAGG17250.070.625681
AGATAGG17300.069.062081
AGTAGGG44500.068.963992
TATAGGG23200.068.672192
ATGTAGG8800.068.419411
TAAGGGA21800.068.339533
AAGGGTA19650.067.68514
AGGGATG34550.067.60485
TAGGGTC8950.067.213444
GATAGGG39350.067.001932
GGTAAGG16850.066.719081
TAGGGCA12750.066.717164
AATAGGG39750.066.682392
GCATAGG7000.066.525661
ATAGGGT11750.066.3956153
GTAGGGA20100.066.169763
Produced by FastQC (version 0.11.5)