FastQCFastQC Report
Mon 27 Feb 2023
SRR3128089.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128089.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3527076
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT507101.4377348262413399No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT56920.16138013470648208No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT144650.085.298531
ACGGGTA4950.075.008044
ATAGGGA29550.071.889733
AGGGTAC19650.071.276415
AGTAGGG57800.070.416752
ATAGGGC18500.068.592713
AGGGATG44300.068.4302445
AGATAGG22250.068.074981
GTAGGGT15400.068.056573
TAGAGGG64700.067.410832
GTAGGGA24150.067.1410143
TAGGGTC9900.066.462824
TAGGGCA18500.066.306294
AAGAGGG121300.066.25542
AGGGAAT33050.066.1262055
ATGTAGG11600.066.098451
TAAGGGA29950.065.908043
GAATAGG20050.065.690921
CGTAGGG9050.065.434672
TATAGGG27600.065.38952
Produced by FastQC (version 0.11.5)