FastQCFastQC Report
Mon 27 Feb 2023
SRR3128088.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128088.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2253092
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT954464.23622293275197No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT88070.39088505928741485No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT87690.38919848812210067No Hit
CGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT84500.3750401670238055No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG48490.21521535738442993No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTC41660.18490145986049394No Hit
CGTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC36910.16381932029406698No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC30240.1342155579976317No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26070.1157076586308948No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22620.10039536778791101No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT261950.090.381181
CGTTTCT13400.073.749081
AGGGAAT25000.072.1878745
ATAGGGC13100.071.034113
AGGGTAC8800.069.9619145
AAGGGTA14250.069.9188164
AGGGATG32100.069.690725
GTAGGGT8900.068.647763
AGTAGGG34100.068.517322
GTAGGGA15550.068.304783
GTACGGG4350.068.084722
TAGGGTA7400.067.9555744
GAGGGAT24650.067.874414
ACGGGTA2150.067.763574
AGAGGGC29650.067.682473
ATAGGGA16900.067.297933
TAGACGG3600.066.666821
TAAGGGA15750.066.542233
CGTAGGG6750.066.163452
GTTTTTT361100.065.992282