FastQCFastQC Report
Mon 27 Feb 2023
SRR3128087.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128087.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2109475
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT877844.161414569975943No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT99420.47130210123371935No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT95480.45262446817336066No Hit
CGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT90970.4312447409900568No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG53590.2540442527169082No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTC43920.20820346294694175No Hit
CGTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC39080.1852593654819327No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC33470.15866507069294492No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23860.1131087118832885No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGT21900.10381730051316085No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT235800.090.563471
ACGGGTA1800.080.937384
ATAGGGC13500.072.060373
AGGGATG26500.070.229495
CGTTTCT13550.070.12181
AGTAGGG31250.069.478742
TAGGGCA8600.069.4009254
ATAGCGG3000.068.987821
AGATAGG10950.068.730081
GTAGGGT7800.068.686323
AGAGGGC26200.068.1619953
ATGTAGG5250.068.091871
AAGGGTA12950.067.862814
GTAGGGA13950.067.7145163
ATAGGGA17500.067.405553
AGGGAAT22800.066.7852255
TAGCGGG8450.066.739742
GTTTTTT323150.066.3308942
CGTAGGG5850.065.8745962
GATAGGG27800.065.760452