FastQCFastQC Report
Mon 27 Feb 2023
SRR3128085.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128085.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1375291
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129870.9443092407352335No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC70800.5148001404793603No Hit
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG53920.39206247986789705No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG50130.3645046757377166No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC47650.34647212844408926No Hit
AGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT20260.14731427748745538No Hit
AAAGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTT19410.14113376732633312No Hit
CGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT16960.12331935568545131No Hit
TAGACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCT16830.12237410119022084No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGGG16450.11961104958877794No Hit
AGAGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTT15630.11364867508040118No Hit
GGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT15280.11110375913170377No Hit
ACTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG14020.10194206171639311No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACG13900.086.557233
AGGGATG40600.086.008425
GGATGGC34700.083.702267
CGTTTTT42800.083.123731
GCTACGA14500.082.9755554
CGTAGGG3350.082.8024752
AGGGAAT27600.082.246285
TAGGGCG1950.081.945014
ACGACCA14500.080.706697
AGGGCGT2100.080.567795
GATGGCA33450.080.3670658
TCCGCTA14950.080.2686541
TAAGCGG2600.079.63911
CGGGATG7100.079.433025
AGAGGGC16550.078.6610953
ATGGCAC33650.078.63249
GAGGGAT27400.078.386964
CTACGAC15350.078.380815
TAGCGGG4700.078.024842
GGGATGG40850.077.888776