FastQCFastQC Report
Mon 27 Feb 2023
SRR3128084.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128084.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1285467
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115320.8971058766969514No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC63430.49343934927928923No Hit
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG58400.45430960110216756No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG54080.42070313745899357No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC53690.41766922060231804No Hit
AGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT22090.17184416247169315No Hit
AAAGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTT21160.164609437659621No Hit
TAGACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCT18920.1471838639187159No Hit
CGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT18820.14640593651956837No Hit
AGAGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTT16570.12890257003874858No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGGG16160.1257130677022436No Hit
GGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT15810.1229903218052272No Hit
ACTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG15790.1228347363253977No Hit
ACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT14660.11404415671503042No Hit
TCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG13280.10330875860679427No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACG12600.088.02413
CGTTTTT38200.087.040291
TAGGGCG1250.086.472484
AGGGATG36750.086.319035
TCCGCTA13150.084.759211
GCTACGA13100.084.66444
ACGGGTA2400.084.201024
CTACGAC13300.083.7446065
AACGGGT3400.081.5517353
AAGGGTA11700.081.5399254
GGATGGC31700.081.097057
TAAGCGG2350.080.0490951
AGGGAAT24800.079.779365
CGGGATG7600.079.769395
GATGACC17950.079.595078
AGAGGGC14600.079.506793
CAGGGTA4350.078.8667144
ATGGCAC29800.078.855289
GATGGCA30400.078.84498
TTACGGG2150.078.6908342
Produced by FastQC (version 0.11.5)