FastQCFastQC Report
Mon 27 Feb 2023
SRR3128083.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128083.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547654
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175581.1344913010272322No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC82310.5318372194301827No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG57150.3692685832879959No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC56660.366102500946594No Hit
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG56040.3620964375758406No Hit
AAAGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTT22280.14395982564578388No Hit
AGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT20880.13491387609892133No Hit
TAGACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCT19240.12431719234402522No Hit
CGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT17550.11339743896245542No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGGG17040.11010212877038408No Hit
ACTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG16830.1087452363383547No Hit
AGAGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTT15720.10157309062619939No Hit
ACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT15630.10099156529818681No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG2700.087.114261
CGTTTTT53100.086.1988141
AGGGATG47200.085.634035
GCTACGA14950.083.623894
CGCTACG15000.082.71853
GGATGGC39450.082.203827
GATGGCA36950.081.533028
TAGCGGG6150.081.009262
CTACGAC15500.080.656595
AGTAAGG8300.080.480971
AGAGGGC18500.080.2796253
TAAGGGA17250.079.830433
CGGGATG8950.079.294655
CGTAGGG5000.078.961112
AAGGGCA20100.078.799564
ACGACCA15450.078.788217
ATGGCAC37850.078.725129
AGGGTAG8500.078.516225
AAGGGAT42000.078.33194
AAGGGTA14350.078.277324