FastQCFastQC Report
Mon 27 Feb 2023
SRR3128082.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128082.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1209367
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120580.9970505231249075No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG39870.3296765994110969No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG37470.3098315068957562No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC35260.2915574842045467No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC16680.13792339298161765No Hit
AGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT14150.11700335795502936No Hit
CGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT13620.11262090002455831No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT38350.085.289261
CGAGGGA6650.079.155013
CGAAGGG10400.079.132732
CGTAGGG3050.078.636062
TAGGGCG1700.077.408944
AGGGATG18700.076.654965
ACGGGTA2350.075.997234
TACGGGT1250.075.197253
GGGCGAT6150.074.891576
AAGGGTA14650.074.748124
GTAGGGT7050.074.663943
AACGAGG5550.074.621611
TAGCGGG5550.074.566052
AAGGGCG5550.074.51984
AGGGTAC10100.074.452725
AGGGAAT18900.074.351785
GGGTACC6200.073.529586
TAGGGTC4800.073.4348144
AGTAAGG6800.073.362121
TAGAGGG21700.072.8166962
Produced by FastQC (version 0.11.5)