FastQCFastQC Report
Mon 27 Feb 2023
SRR3128080.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128080.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1404437
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164151.1687957523192567No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG41930.2985537977139594No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG40950.29157591262548627No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC35940.25590325518339374No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC21140.15052294976563563No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15480.11022210323424975No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT49200.082.013931
ATAGCGG2800.080.621081
CGAAGGG12550.080.5159152
TAGCGGG6450.080.153032
AGGGATG23750.079.359465
AGGGAAT22300.078.828765
AGGGTAC10850.077.975955
CGTAGGG4100.077.949272
ACGGGTG3200.077.8418054
GGGCGAT5100.077.4153446
TACGGGT1400.077.212363
AAGGGTA15550.077.072034
TAAGCGG2750.076.956491
AGGGTAA9800.075.7790155
ACGTAGG1700.074.693061
AGGGTAT11950.074.3381655
TAGGGTA8050.074.147214
TAGGGTC6550.073.906554
GAGGGTA12650.073.5633854
TAGAGGG24300.072.915872
Produced by FastQC (version 0.11.5)