FastQCFastQC Report
Mon 27 Feb 2023
SRR3128078.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128078.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164451
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110360.9477427560283772No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG40750.3499503199361759No Hit
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC39980.3433377617435169Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG39650.3405038082323773No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36800.3160287551816264No Hit
CGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT15060.12933133296291557No Hit
AAAGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTT14690.12615386993527422No Hit
AGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT14600.12538097352314523No Hit
GGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT12360.10614444059904625No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAGG1950.086.817971
CGTTTTT33750.086.807261
AGGGATG39700.083.811715
GATGGCA31800.081.578168
GGATGGC34600.081.088677
GGTAAGG6050.080.8387761
TACGCGG353.849709E-880.616691
CGAAGGG9300.079.846292
AGTACGG650.079.5831451
ACGGGTA1300.079.531894
CGTAGGG3800.079.154752
CGAGGGA6650.079.154753
GATGACC19100.078.9838
CAGGGAT14000.078.886334
AGGGAAT24150.078.618765
TAAGCGG2650.078.081571
TAGGGCG1450.077.7866744
GAGGGAT23050.077.273444
GAGGGTA8350.077.10744
AAGGGCA15350.076.54094
Produced by FastQC (version 0.11.5)