FastQCFastQC Report
Mon 27 Feb 2023
SRR3128077.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128077.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1437575
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158881.1051945115906996No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC46280.3219310296854077No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG42900.2984192129106307No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG42330.2944542023894406No Hit
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC38330.2666295671530181Illumina Single End Adapter 1 (95% over 21bp)
AAAGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTT16290.11331582700033042No Hit
CGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT15350.10677703771977115No Hit
AGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT14380.10002956367493869No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGG600.086.246061
CGTTTTT50050.085.7212751
AGGGATG50100.082.2719355
CGTAGGG3950.082.099752
GGATGGC44650.081.68277
GATGGCA42300.080.4429558
CGAAGGG11100.080.025542
ACGGGTA2200.079.043994
ATGGCAC42500.078.737379
TAGGGCA7800.078.331894
GATGACC22600.078.1932458
CGAGGGA9950.077.9382553
TAGGGCG2850.077.507344
TTACGGG2550.077.410342
CGCTACG9050.077.379783
AGGGAAT29850.076.678655
CAGGGAT16450.076.5700154
AGGGTAT10050.076.227455
GCGGGAT9350.075.9023364
CGGGTAA1800.075.7208255