FastQCFastQC Report
Mon 27 Feb 2023
SRR3128076.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128076.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2584886
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241840.9355925174263005No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT52750.2040708951961518No Hit
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG51930.20089860829452438RNA PCR Primer, Index 27 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG42790.16553921526906795RNA PCR Primer, Index 38 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC35910.13892295443590164RNA PCR Primer, Index 38 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70900.082.249891
GTACGGG5000.081.81142
TAGGGCA13300.075.972794
AAGGGTA22250.072.449514
ATAGGGA20300.072.000533
AGTAGGG39150.071.577922
TAAGGGA21150.070.884553
AGGGTAC12950.070.0420465
ATAAGGG35700.069.80272
AGTACGG2850.069.360891
AGGGACT41450.069.1634455
GTAGGGA20500.068.776293
GAATAGG15100.068.573451
AGGGTAT14700.068.417595
AGGGATG33350.068.346655
ATAGGGC12950.068.2274863
AGGGAAT32250.068.200485
ATGAGGG39200.067.408532
AGGGCAT21800.067.261955
CGTAGGG7200.067.2619252
Produced by FastQC (version 0.11.5)