FastQCFastQC Report
Mon 27 Feb 2023
SRR3128075.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128075.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2409292
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218240.9058262759350049No Hit
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG57860.240153538881962RNA PCR Primer, Index 27 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT48800.2025491306159652No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG46340.1923386621463899RNA PCR Primer, Index 38 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC39810.16523526413568798RNA PCR Primer, Index 38 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT65200.081.504031
AGGGTAC12500.076.324465
TAGGGCA11650.075.030474
CGTAGGG6800.074.638952
ATAGGGA19500.073.504843
AGTTAGG8500.073.030321
AGGGAAT30550.071.842825
ATAAGGG33200.071.200092
TAGGGTA8850.071.156114
ATAGCGG4650.069.782151
AGTAGGG36450.069.750992
TAGGGTC8200.068.773024
GTAGGGT10250.068.314533
TAAGGGA17800.067.852183
TGTAGGG17000.067.7279362
GAGGGTA13900.067.618554
GTAGGGA21250.067.230353
AGGGATG28700.066.975985
AAGGGTA18900.066.638264
AGGGTAT13200.066.5802465
Produced by FastQC (version 0.11.5)