FastQCFastQC Report
Mon 27 Feb 2023
SRR3128074.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128074.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2962268
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT317401.0714763147696291No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT61810.20865769066134462No Hit
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG55320.18674880193149304RNA PCR Primer, Index 27 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG47480.16028259428248895RNA PCR Primer, Index 38 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC39970.13493039792483327RNA PCR Primer, Index 38 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT90400.082.343151
TAAGGGA25550.072.293573
ATAGGGA23100.070.601763
ACGGGTA3550.070.169044
AGGGTAC15350.069.808755
CGTAGGG8450.069.526652
AGGGAAT34850.069.182765
ATAAGGG43250.068.571122
GAATAGG18500.068.4179461
GTACGGG6550.068.167962
GAGGGAT29400.067.462614
AGTAGGG48300.067.240192
AGGGACT50000.066.925765
TAGGGCA15250.066.8787164
ATGAGGG44900.066.6793062
AGGGATG36400.066.624155
TAGGGTA12300.066.487834
TGTAGGG22250.066.328112
TATAGGG20250.066.148172
GTAGGGA27800.065.5971453
Produced by FastQC (version 0.11.5)