FastQCFastQC Report
Mon 27 Feb 2023
SRR3128073.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128073.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2156624
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209940.9734659356475677No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29490.13674149967727336No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29310.13590686183590647No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG22940.106369956005312No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64250.083.133191
TAGGGTA9650.075.486934
ACGGGTC3850.074.4622964
ACGGGTA2850.074.205314
CGTAGGG6350.074.038012
ATAGAGG14850.070.3522951
GTAGGGA18750.070.181733
TAAGGGA20500.069.6926653
AGTAGGG36850.068.7669452
GTACGGG4150.067.9722442
AAGGGTA18450.067.756754
ATAGGGC11400.067.6092763
ATAGCGG4050.067.394541
TAGGGCA11450.067.314044
AGGGTAT13250.067.0368045
AGGGTAC10800.067.01385
AAGAGGG75950.066.4202352
AGAGGGC30500.066.411723
TAGCGGG10700.066.3470462
GTAGGGT10750.066.013973
Produced by FastQC (version 0.11.5)