FastQCFastQC Report
Mon 27 Feb 2023
SRR3128072.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128072.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2050479
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196390.9577762074129995No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27470.1339686970702943No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27190.1326031624805716No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG25610.1248976458671364No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT63550.084.753281
ACGGGTA2150.072.130394
TATAGGG15200.070.491062
CGTAGGG7250.069.356722
ATAGGGC12850.068.388213
ATAGAGG15900.068.341011
AGTAGGG33300.067.880282
TAGGGTA8650.067.367174
ACTAGGG10150.067.134342
ACGGGTC3650.066.950414
GGGCGAT10350.066.750046
AGGGCAT16350.066.40015
TAGGGCA11500.066.2002944
GAATAGG12900.065.2724461
TAAGGGA20400.065.192713
AAGGGTA15650.065.1610644
GAGGGTA14900.064.656234
AGGGATG28000.064.453685
AAGAGGG67900.064.0890662
GTAGGGT10500.064.00143
Produced by FastQC (version 0.11.5)